Question

Which of the following is not true for covalent catalysis?

(a) A number of coenzymes for covalent adducts generating new electrophilic groups which can function as electron sink

(b) These adduct forming coenzymes leads to increase in rate acceleration

(c) About 100 enzymes show covalent intermediates during catalysis

(d) Hydrolysis of acetyl imidazole is an example of this type of catalysis

This question was posed to me in my homework.

The query is from Mechanism of Enzyme Catalysis in section Enzyme Assay & Enzyme Catalysis of Enzyme Technology

Answer 1

The correct choice is (d) Hydrolysis of acetyl imidazole is an example of this type of catalysis

To elaborate: Hydrolysis of acetyl imidazole is an example of acid base catalysis, and not covalent catalysis. The following statements are true for covalent catalysis:

• A number of coenzymes for covalent adducts generating new electrophilic groups which can function as electron sink.

• These adduct forming coenzymes leads to increase in rate acceleration by route low energy, stabilized, substrate derived carbonions.

• About 100 enzymes show covalent intermediates during catalysis.