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Which of the following enzyme is assayed and measured using radioisotope method?

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Which of the following enzyme is assayed and measured using radioisotope method?

(a) Hydrolases

(b) Bacterial luciferase

(c) Phosphofructokinase

(d) Glutamate decarboxylase

The question was asked during an interview for a job.

My question is based upon Enzyme Assay Techniques topic in division Enzyme Assay & Enzyme Catalysis of Enzyme Technology

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Right choice is (d) Glutamate decarboxylase

To explain I would say: Glutamate decarboxylase is assayed and measured using radioisotope method. This assay is based on evolution ^14CO2. Then the evolved ^14CO2 is trapped in alkali and then measured. Phosphofructokinase is assayed and measured using spectrophotometer. Hydrolases is measured by using spectrofluorometric method, whereas bacterial luciferase is measured by using luminescence method.

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