Question

Which of the following is not true for luminescence method?

(a) The intensity of emitted light is used to study enzyme reaction

(b) The reaction can be used to assay ATP and appropriate enzymes via coupled reaction

(c) Bacterial luciferase can be assayed and measured by this method

(d) It is based on stoke shift

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The question is from Enzyme Assay Techniques topic in portion Enzyme Assay & Enzyme Catalysis of Enzyme Technology

Answer 1

The correct option is (d) It is based on stoke shift

The explanation is: “It is based on stoke shift.” It is true for spectrofluorometric method, and not luminescence method. Statements which are true for luminescence method are:

• The intensity of emitted light is used to study enzyme reaction.

• The reaction can be used to assay ATP and appropriate enzymes via coupled reaction.

• Bacterial luciferase can be assayed and measured by this method.