Question

Continuous assay: Glucose-6-phosphate dehydrogenase:: Luminescence method: ____________

(a) Hydrolases

(b) Bacterial luciferase

(c) Ornithine decarboxylase

(d) Glutamate decarboxylase

I got this question during an internship interview.

Query is from Enzyme Assay Techniques in section Enzyme Assay & Enzyme Catalysis of Enzyme Technology

Answer 1

Right choice is (b) Bacterial luciferase

To explain: Glucose-6-phosphate dehydrogenase can be assayed and measured by using continuous assay. In the similar way, Bacterial luciferases can be assayed and measured using luminescence method. This enzyme reduces FMN to oxidizing long chain aliphatic aldehydes. The resulting FMN can be coupled with NAD(P)H.