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The reaction catalyzed by ornithine decarboxylase is an example of which assay?

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The reaction catalyzed by ornithine decarboxylase is an example of which assay?

(a) Continuous assay

(b) Coupled assay

(c) Enzymatic assay technique

(d) Discontinuous assay

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This interesting question is from Continuous, Discontinuous and Coupled Assay topic in portion Enzyme Assay & Enzyme Catalysis of Enzyme Technology

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Correct option is (b) Coupled assay

The best explanation: The reaction catalyzed by ornithine decarboxylase is an example of coupled assay. In this assay, L-[1-C^14] ornithine is used which liberates radioactive CO2 which can be trapped by a suitable base such as ethanolamine dissolved in 2-methoxyethanol. Then the radioactivity is measured by scintillation counting.

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