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Which of the following is true for radioisotope method?

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Which of the following is true for radioisotope method?

(a) The intensity of emitted light is used to study enzyme reaction

(b) The reaction can be used to assay ATP and appropriate enzymes via coupled reaction

(c) It is restricted to separation of radiolabeled forms of substrate and product

(d) Bacterial luciferase can be assayed and measured by this method

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My enquiry is from Enzyme Assay Techniques topic in portion Enzyme Assay & Enzyme Catalysis of Enzyme Technology

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The correct answer is (c) It is restricted to separation of radiolabeled forms of substrate and product

Best explanation: The statement true for radioisotope method is “It is restricted to separation of radiolabeled forms of substrate and product.” The other following statements are true for luminescence method:

• The intensity of emitted light is used to study enzyme reaction.

• The reaction can be used to assay ATP and appropriate enzymes via coupled reaction.

• Bacterial luciferase can be assayed and measured by this method.

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