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In which of the following assays, there is a “stop and sample” procedure?

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in Enzyme Technology by (55.0k points)
In which of the following assays, there is a “stop and sample” procedure?

(a) Continuous assay

(b) Coupled assay

(c) Enzymatic assay technique

(d) Discontinuous assay

This question was addressed to me in semester exam.

Enquiry is from Continuous, Discontinuous and Coupled Assay in section Enzyme Assay & Enzyme Catalysis of Enzyme Technology

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Right choice is (d) Discontinuous assay

To elaborate: In discontinuous assay, there is a “stop and sample” procedure which is used. Hence the name. This means that taking the sample out at required time and stopping the enzymatic activity and then measuring the amount of product. This procedure has a disadvantage of sampling errors and estimation of product. But this can be corrected by use of ion-exchange chromatography.

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