Question

Which of the following enzyme is assayed and measured using spectrofluorometric method?

(a) Glucose-6-phosphate dehydrogenase

(b) Hydrolases

(c) Phosphofructokinase

(d) Glutamate decarboxylase

This question was posed to me in an international level competition.

This is a very interesting question from Enzyme Assay Techniques in chapter Enzyme Assay & Enzyme Catalysis of Enzyme Technology

Answer 1

Correct option is (b) Hydrolases

Explanation: Hydrolases can be assayed by measuring the rate of appearance of fluorescence at 450nm of the anion 4-methylumbelliferone. Phosphofructokinase is assayed and measured using spectrophotometer. Hydrolases is measured by using spectrofluorometric method, whereas bacterial luciferase is measured by using luminescence method. Glucose-6-phosphate dehydrogenase can be assayed by using continuous assay.