The correct option is (c) Measure the convenient change in absorbance
The best explanation: “Measure the convenient change in absorbance.” This step is involved in continuous assay, and not discontinuous, which has “stop and sample procedure”. The steps involved in discontinuous assay are as follows:
• Taking sample from the reaction mixture at known time after addition of enzyme.
• Stopping the reaction by addition of acid to inactivate the enzyme.
• Measuring the amount of product formed.