Question

Which of these factors is not true for enzymes while controlling assays?

(a) Extreme high salt concentration cannot be tolerated

(b) pH (6-8) is suitable for maximum activity

(c) Decrease in temperature leads to decreased reaction rates

(d) Increase in substrate concentration leads to increase in rate of reaction

I had been asked this question during a job interview.

This interesting question is from Factors Controlling in Assays topic in chapter Enzyme Assay & Enzyme Catalysis of Enzyme Technology

Answer 1

Correct option is (c) Decrease in temperature leads to decreased reaction rates

The best explanation: “Decrease in temperature leads to decreased reaction rates.” This is not true. All enzyme work within a specific range of temperature which is suitable to the organisms. Increase in temperature leads to increase in reaction with a limitation that, at higher temperature, there might be decrease in reaction rates.