Question

Which of these factors is true for enzymes while controlling assays?

(a) Extreme high salt concentration can be tolerated

(b) pH (6-8) is suitable for maximum activity

(c) Decrease in temperature leads to decreased reaction rates

(d) Decrease in substrate concentration leads to increase in rate of reaction

The question was asked during an internship interview.

Question is taken from Factors Controlling in Assays topic in division Enzyme Assay & Enzyme Catalysis of Enzyme Technology

Answer 1

Correct option is (b) pH (6-8) is suitable for maximum activity

For explanation I would say: “pH (6-8) is suitable for maximum activity”. This statement is true. Most enzymes are sensitive to pH and have specific range activity of 6-8. Enzyme activity can be stopped due changes in pH which leads denaturation of 3-dimensional structure of enzyme by breaking the ionic and hydrogen bonds present in it.