Right answer is (c) The probe must be tiny and biocompatible
Best explanation: “The probe must be tiny and biocompatible.” This is feature of biosensor, and not precautions to be followed. Following are the precautions that must not be followed while performing assays:
• The substrates, buffers etc., should be of high purity as the contaminants may affect the activity of the enzyme. For example: Certain NAD^+ preparations contain inhibitor of dehydrogenases.
• Enzyme preparation should as pure as possible so that any other compounds or enzyme will not interfere with assay
• Stability of the enzyme during the time taken by assay to complete the reaction.
• pH, temperature etc., affects the activity of the enzyme, and hence has to be maintained by use of buffers, thermostable bath etc.
• It should be ensured that once steady state is reached, the rate of reaction is constant over a period of interest and proportional to the amount of enzyme added.