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Which of the following assays, there is a convenient change is absorbance?

(a) Continuous assay

(b) Coupled assay

(c) Enzymatic assay technique

(d) Discontinuous assay

I had been asked this question in an interview for job.

The query is from Continuous, Discontinuous and Coupled Assay topic in division Enzyme Assay & Enzyme Catalysis of Enzyme Technology

1 Answer

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Right option is (a) Continuous assay

To explain: In continuous assays, convenient change in absorbance is seen. Discontinuous assay is one which has a “stop and sample procedure. Coupled assays are those which involve coupling of enzyme and substrate such that coupling reaction is not limiting one. Enzymatic assay technique are the procedures used to measure the amount of substrate or product in all the 3 types of assays.

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