Question

Use of thermostable baths, buffers are necessary while performing assays.

(a) True

(b) False

I had been asked this question at a job interview.

My doubt stems from Continuous, Discontinuous and Coupled Assay in portion Enzyme Assay & Enzyme Catalysis of Enzyme Technology

Answer 1

Correct choice is (a) True

Explanation: The enzyme activity is affected by changes in temperature, pH, etc. To ensure that these parameters are stabilized, use of thermostable bath, buffer becomes necessary. Hence the above statement is true.