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Macromolecular crowding does alter the rate and equilibrium constant of enzyme reaction.

(a) True

(b) False

I have been asked this question in my homework.

This interesting question is from Factors Controlling in Assays in section Enzyme Assay & Enzyme Catalysis of Enzyme Technology

1 Answer

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The correct option is (a) True

Easiest explanation: Macromolecular crowding occurs because of the large amount of macromolecule in solution. The large amounts lead to cellular destruction by a natural process which is accompanied by changes in enzymes and breakdown of cofactors. This leads to the death of the organisms, in turn affecting the rate and equilibrium constant of enzyme reaction.

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