Question

What does the following diagram represent?

(a) Effect of substrate inhibition on the rate of enzyme catalyzed reaction

(b) Effect of multipoint interaction for stabilization of enzyme

(c) Miachelis Menten plot

(d) Effect of temperature on activity of enzyme

The question was asked in class test.

This intriguing question comes from Kinetics of Immobilized Enzymes topic in section Immobilized Enzymes Preparation and Kinetics of Enzyme Technology

Answer 1

Right option is (b) Effect of multipoint interaction for stabilization of enzyme

Best explanation: The above diagram represents the multipoint interaction for stabilization of enzyme. Point ‘a’ represents the activity of the free enzymes whereas point ‘b’ represent the activity of the enzyme bound to some matrix. Point ‘c’ and ‘d’ represent the activity of the bound enzyme covalently and activity of the entrapped enzyme in a gel respectively. Miachelis Menten plot turns out to be a hyperbolic curve. Effect of temperature on activity of enzymes turns out to be a bell shaped curve.