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What does the equation, λ=\(\frac{[C_0^{n+}]}{[C^{n+}]}=\frac{[A^{n-}]}{[A_0^{n-}]}\) represent?

(a) Turnover number

(b) Catalytic efficiency

(c) Electrostatic potential coefficient

(d) Porosity

I have been asked this question in semester exam.

The doubt is from Kinetics of Immobilized Enzymes topic in chapter Immobilized Enzymes Preparation and Kinetics of Enzyme Technology

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Correct choice is (c) Electrostatic potential coefficient

The best explanation: The electrostatic potential coefficient is given by λ=\(\frac{[C_0^{n+}]}{[C^{n+}]}=\frac{[A^{n-}]}{[A_0^{n-}]}\) where,

\(C_0^{n+}\) and \(A_0^{n-}\) ae concentration of anions and cations in bulk environment

C^n+ and A^n- are concentration of anions and cations in microenvironment

For positively charged enzyme surface, the value of λ should be greater than 1 and negatively charged the value should be less than 1. The effect of partition on positively and negatively charged particles is equal and opposite. Kcat is referred to as turn over number that denotes the number of catalytic turn over events that happens per unit time. Kcat/ Km is termed as catalytic efficiency. Porosity is referred to as the ratio of volume of solution contained within the particle to the total volume of the particle.

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