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Immobilized enzymes observe diffusional resistance at different levels.

(a) True

(b) False

This question was posed to me in an internship interview.

My enquiry is from Kinetics of Immobilized Enzymes in chapter Immobilized Enzymes Preparation and Kinetics of Enzyme Technology

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The correct answer is (a) True

Explanation: In the reaction catalyzed by immobilized enzymes, the substrates diffuses through the solutions to the catalytic active sites and products diffuse away in the bulk solutions. This diffusion is due to the concentration gradient created as solutes move in direction of higher to lower concentration. At different levels in immobilized enzymes observe diffusional resistance. This resistance may vary depending on

The nature of support material

Hydro-dynamical conditions surrounding the support material

Distribution of enzyme inside/ on the surface of the support material.

Hence the above statement is true.

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