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Immobilization affects the kinetic constants (Km and Vmax) of the enzyme.

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Immobilization affects the kinetic constants (Km and Vmax) of the enzyme.

(a) True

(b) False

I had been asked this question in an interview for job.

I want to ask this question from Kinetics of Immobilized Enzymes in portion Immobilized Enzymes Preparation and Kinetics of Enzyme Technology

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The correct option is (b) False

Easy explanation: The above statement is true. For example: Trypsin in free form hydrolyses 15 peptides of pepsinogen whereas trypsin in immobilized form hydrolyses 10 bonds. This is due to change in properties of solution in the immediate vicinity of immobilized enzymes or due to molecular diffusion within local environment.

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