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What does the following graph predicts?

(a) The effect of diffusional control on the local pH

(b) The effect of diffusional control on pH activity profile of an immobilized enzyme

(c) Effect of substrate inhibition

(d) Arrhenius plot

This question was addressed to me during an internship interview.

My enquiry is from Kinetics of Immobilized Enzymes topic in division Immobilized Enzymes Preparation and Kinetics of Enzyme Technology

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The correct option is (b) The effect of diffusional control on pH activity profile of an immobilized enzyme

Easiest explanation: The above graph predicts the effect of diffusional control on pH activity profile of an immobilized enzyme. This graph shows the effect on surface on the pH activity profile. The proton modulus (µH) and the pH activity profile varies with flowrate and degree of turbulence. If the reaction is started at pH = 10 and µH = 0.1, the rate of hydrogen ion production is initially low, but its faster than the rate of diffusion of hydrogen ions. Due to which, there is a drop in the micro-environmental pH towards the optimal pH, increasing the rate of reaction. This process continues until the rate of diffusion equals the rate of reaction.

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