Ask a Question

Which of the following equation is true for \(K_m^{app}\), when the substrates are negatively charged?

← Prev Question Next Question →
+1 vote
in Enzyme Technology by (55.0k points)
Which of the following equation is true for \(K_m^{app}\), when the substrates are negatively charged?

(a) Kcat/Km

(b) Km/λ

(c) Kmλ

(d) Vmax/2

This question was addressed to me in a job interview.

The origin of the question is Kinetics of Immobilized Enzymes in division Immobilized Enzymes Preparation and Kinetics of Enzyme Technology

1 Answer

+2 votes
by (727k points)
selected by
 
Best answer
The correct choice is (b) Km/λ

Easiest explanation: \(K_m^{app}\) = Km/λ for negatively charged substrates. \(K_m^{app}\) is found to be less than Km when the concentration of substrate in the vicinity of the enzyme active site is more than that in bulk solution. This is because Km is maximum at lower bulk concentration. Kcat/Km is the catalytic efficiency. Kmλ is the \(K_m^{app}\) for positively charged substrates. Km is the substrates concentration at which Vmax is reduced to half.

Related questions

We welcome you to Carrieradda QnA with open heart. Our small community of enthusiastic learners are very helpful and supportive. Here on this platform you can ask questions and receive answers from other members of the community. We also monitor posted questions and answers periodically to maintain the quality and integrity of the platform. Hope you will join our beautiful community

Categories

Powered by TalkJarvis
...