Correct option is (a) Kmλ

To explain: For immobilized enzymes, the Miachelis Menten equation changes to be \(V=\frac{V_max}{1+\frac{K_m^{app}}{[S_0]}}\)

\(K_m^{app}\) is apparent Km and given \(K_m^{app}\) = Kmλ for positively charged substrates. This can be calculated when the concentration of positively charged substrates in the bulk solution is known. For negatively charged substrates, the \(K_m^{app}\) = Km/λ. Km is the substrate concentration at which Vmax is reduced to half. Vmax is the maximum rate equation.