Question

Which of the following is a precaution which must be followed while performing assays?

(a) It should be ensured that once steady state is reached, the rate of reaction is constant over a period of interest and proportional to the amount of enzyme added

(b) The response should be accurate, reproducible, precise, linear and free from electrical noise

(c) The probe must be tiny and biocompatible

(d) It should be cheap, small portable and capable of being used by semi-skilled operators

I got this question by my college professor while I was bunking the class.

My doubt is from Continuous, Discontinuous and Coupled Assay in section Enzyme Assay & Enzyme Catalysis of Enzyme Technology

Answer 1

The correct option is (a) It should be ensured that once steady state is reached, the rate of reaction is constant over a period of interest and proportional to the amount of enzyme added

For explanation: The precaution to be taken while performing assay is “it should be ensured that once steady state is reached, the rate of reaction is constant over a period of interest and proportional to the amount of enzyme added.”  Following are the beneficial features of biosensor:

• The response should be accurate, reproducible, precise, linear and free from electrical noise.

• The probe must be tiny and biocompatible.

• It should be cheap, small portable and capable of being used by semi-skilled operators.