Question

Which type of specificity is represented in the following reaction?

Glucose \(_{\overrightarrow{Glucokinase}}\) Glucose-6-phosphate

(a) Relative substrate specificity

(b) Absolute substrate specificity

(c) Broad substrate specificity

(d) Stereo specificity

I have been asked this question in a job interview.

I would like to ask this question from Specificity of Enzyme Action in section Enzymes of Enzyme Technology

Answer 1

Correct choice is (b) Absolute substrate specificity

Explanation: Absolute substrate specificity is one in which the enzymes will act on only substrate and catalyze only one reaction. Thus, glucose converted to glucose 6 phosphate by glucokinase is an example for absolute specificity. Relative substrate specificity is one in which the enzyme acts on more than one substrate. Broad substrate specificity is one in which the enzyme acts on more than one structurally related substrates. The specificity in which the enzymes show specificity towards only one type of isomers is known as stereo specificity.