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Which of the following is an example for broad substrate specificity?

(a) Chymotrypsin hydrolyzing peptide bonds attached to aromatic aminoacids

(b) D-glucose oxidase acting only on D-glucose and not L-glucose

(c) Hexokinase phosphorylating one or more kind of hexoses

(d) Lipase hydrolyzes ester bond of lipids

The question was asked in semester exam.

My doubt is from Specificity of Enzyme Action topic in chapter Enzymes of Enzyme Technology

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Right answer is (c) Hexokinase phosphorylating one or more kind of hexoses

Easiest explanation: The specificity in which the enzyme acts on more than one structurally related substrates is referred to as broad substrate specificity. The example for this type of specificity is hexokinase phosphorylating one or more kind of hexoses which include glucose, mannose fructose etc.

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