Question

The covalent catalysis is aided by one of the following method?

(a) General acid-base catalysis

(b) Specific acid-base catalysis

(c) Nucleophilic catalysis

(d) Substrate collision theory

This question was addressed to me in an interview for internship.

I would like to ask this question from Enzyme Kinetics topic in chapter Enzyme Kinetics of Enzyme Technology

Answer 1

The correct answer is (c) Nucleophilic catalysis

To explain: Covalent catalysis involves substrates forming transient covalent bond with the residues present in the active site. The covalent catalysis is aided by nucleophilic and electrophilic catalysis. In most of the enzymatic reaction protons are involved. General and specific acid-base catalysis are types of acid-base catalysis. Substrate collision theory says about the binding between substrate and enzyme.