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In the following plot, what does X represent?

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in Enzyme Technology by (55.0k points)
In the following plot, what does X represent?

(a) Vmax

(b) Km/Vmax

(c) –1/Km

(d) Smax

The question was posed to me at a job interview.

Question is from Kinetics of Enzyme Catalysed Reaction topic in portion Enzyme Kinetics of Enzyme Technology

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Right option is (c) –1/Km

Explanation: The plot represents Lineweaver Burk plot which is devised from the equation, \(\frac{1}{V_0} = \frac{K_m}{V_{max}} \frac{1}{[S]}+\frac{1}{V_{max}}\) where,

V0 = initial velocity, Vmax = maximum rate, Km = Miachelis Menten constant and [S] = substrate concentration.

According to this plot, the slope gives Km/Vmax, the y intercept gives 1/Vmax and the X on the x-intercept gives -1/Km.

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