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Which of the following is based on rearrangement of Lineweaver Burk plot equation by multiplication of [So] factor?

(a) Hanes plot equation

(b) Eadie-Hofstee plot equation

(c) Miachelis Menten equation

(d) Eisenthal and Cornish and Bowden plot equation

I got this question by my college professor while I was bunking the class.

Question is from Kinetics of Enzyme Catalysed Reaction topic in division Enzyme Kinetics of Enzyme Technology

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Right answer is (a) Hanes plot equation

To explain I would say: Hanes plot equation is based on rearrangement of the Lineweaver Burk plot equation by multiplication of [S0] factor and is given by \(\frac{[S_0]}{V_0} = \frac{1}{V_{max}} [S_0]+\frac{K_m}{V_{max}}\) where,

V0 = initial velocity, Vmax = maximum rate, Km = Miachelis Menten constant and [S] = substrate concentration.

The graphical representation of [S0]/V0 versus [S0] is called the Hanes plot. One of the drawbacks of this approach is that neither the ordinate nor the abscissa represents independent variable, both are dependent on substrate concentration.

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