Question

Which of these is referred to as Kcat?

(a) Miachelis Menten constant

(b) Catalytic efficiency

(c) Substrate concentration

(d) Turn over number

This question was addressed to me in an interview.

This interesting question is from Kinetics of Enzyme Catalysed Reaction in chapter Enzyme Kinetics of Enzyme Technology

Answer 1

The correct option is (d) Turn over number

For explanation: Kcat is referred to as turn over number which denotes the number of catalytic turn over events that happens per unit time. Catalytic efficiency is given by Kcat/Km. Miachelis Menten constant is given by Km. Substrate concentration is the amount substrate used in an enzyme catalyzed reaction.