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Which of the following matrix is not used while immobilizing the enzyme by covalent binding?

(a) Agarose

(b) Cellulose

(c) Calcium alginate

(d) Glutaraldehyde

This question was addressed to me in quiz.

My question is from Methods of Immobilization topic in section Immobilized Enzymes Preparation and Kinetics of Enzyme Technology

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The correct choice is (c) Calcium alginate

The best I can explain: The enzyme is linked to the carrier matrix by covalent binding between them. This is referred to immobilization by covalent binding. The function groups of the enzyme such as amino-acid group, sulphydryl, imidazole or free carboxyl groups are covalently linked to a carrier matrix. The most commonly used matrix are agarose, cellulose, polyacrylamide, glutaraldehyde, trialkoxysilanes etc. Calcium alginate is used for entrapment method of immobilization.

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