Question

___________ is defined as the enzyme’s binding efficiency with its appropriate substrate through optimized multiple non-covalent interactions.

(a) Km

(b) Kcat

(c) Catalytic efficiency

(d) Vmax

The question was asked in an interview.

I'd like to ask this question from Kinetics of Enzyme Catalysed Reaction topic in division Enzyme Kinetics of Enzyme Technology

Answer 1

Correct answer is (a) Km

For explanation: Km is defined as the enzyme’s binding efficiency with its appropriate substrate through optimized multiple non-covalent interactions. Km is also referred to as the substrate concentration at which the reaction occurs at half of the maximum rate. Kcat is referred to as turnover number that denotes the number of catalytic turn over events that happen per unit time. Catalytic efficiency is referred to Kcat/Km. The high value of this indicates collision frequencies between enzyme and substrate molecule and low value indicates the equilibrium assumption status. Vmax is the maximum rate of the reaction.