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What does the following graph represent?

(a) Miachelis Menten plot

(b) Lineweaver Burk plot

(c) Eadie-Hofstee plot

(d) Hanes plot

I got this question during an online interview.

Asked question is from Kinetics of Enzyme Catalysed Reaction topic in section Enzyme Kinetics of Enzyme Technology

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The correct answer is (d) Hanes plot

The explanation is: Hanes plot is a graphical representation of [S0]/V0 is plotted against [S0] which is based on the equation, \(\frac{[S_0]}{V_0} = \frac{1}{V_{max}} [S_0]+\frac{K_m}{V_{max}}\), where

V0 = initial velocity, Vmax = maximum rate, Km = Miachelis Menten constant and [S] = substrate concentration.

This plot yields a straight line of slope 1/Vmax, a y-intercept of Km/Vmax and an x-intercept of -Km.

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