Question

What does the following plot represent?

(a) Miachelis Menten plot

(b) Hanes plot

(c) Lineweaver Burk plot

(d) Eadie-Hofstee plot

I got this question in class test.

This interesting question is from Kinetics of Enzyme Catalysed Reaction in section Enzyme Kinetics of Enzyme Technology

Answer 1

The correct answer is (a) Miachelis Menten plot

Explanation: Miachelis Menten equation is given by \(V_0 = \frac{V_{max}[S]}{K_m+[S]}\), where

V0 = initial velocity, Vmax = maximum rate, Km = Miachelis Menten constant and [S] = substrate concentration.

According to this equation, Vo versus [S] plot giving a hyperbolic curve is referred to as Miachelis Menten plot. The tangent to the curve at the origin goes through the point (V0 = Vmax), ([S] = Km). The substrate concentration, which gives a rate of half the maximum reaction velocity, is equal to the Km.