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The equation which is based on reciprocal form of Miachelis Menten equation at constant [Et] level is referred to as ______________

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The equation which is based on reciprocal form of Miachelis Menten equation at constant [Et] level is referred to as ______________

(a) Eadie-Hofstee plot equation

(b) Eisenthal and Cornish and Bowden plot equation

(c) Lineweaver Burk equation

(d) Hanes plot equation

I had been asked this question at a job interview.

My query is from Kinetics of Enzyme Catalysed Reaction topic in portion Enzyme Kinetics of Enzyme Technology

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The correct choice is (b) Eisenthal and Cornish and Bowden plot equation

The best explanation: To overcome certain limitation posed by various plots in determining Km and Vmax, the equation based on reciprocal form of Miachelis Menten equation is referred to as Eisenthal and Cornish and Bowden plot equation. The equation is given by \(\frac{V_{max}}{V_0} = \frac{K_m}{[S_0]}+1\) where,

V0 = initial velocity, Vmax = maximum rate, Km = Miachelis Menten constant and [S] = substrate concentration.

At constant V0 and S0, a plot of Vmax against Km will be linear.

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