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_____________ involves donation of electrons from nucleophilic active site residues to a substrate forming a covalent adduct transition state intermediate.

(a) General acid-base catalysis

(b) Specific acid-base catalysis

(c) Electrophilic catalysis

(d) Nucleophilic catalysis

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This interesting question is from Enzyme Kinetics topic in portion Enzyme Kinetics of Enzyme Technology

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The correct choice is (d) Nucleophilic catalysis

Explanation: In most of the enzymatic reaction protons are involved. General and specific acid-base catalysis are types of acid-base catalysis. The catalysis which involves donation of electrons from nucleophilic active site residues to a substrate forming a covalent adduct transition state intermediate is referred to as nucleophilic catalysis. Nucleophilic and electrophilic catalysis are used to aid covalent catalysis.

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