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In the catalysis of RNA by Bovine pancreatic RNase A, His 12 acts as proton donor.

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in Enzyme Technology by (55.0k points)
In the catalysis of RNA by Bovine pancreatic RNase A, His 12 acts as proton donor.

(a) True

(b) False

I got this question in an interview.

Query is from Enzyme Kinetics topic in portion Enzyme Kinetics of Enzyme Technology

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Correct answer is (b) False

The explanation is: In most of the enzymatic reactions, some type of proton transfer is involved. In catalysis of RNA by bovine pancreatic RNase A, His 12 (imidazole) acts as proton acceptor and His 119 acts as a proton donor to attain the transition state. Hence the above statement is false.

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