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In the biphasic system, which of the following methodology is not used to remove the excess water present?

(a) Use of hydrolases

(b) Chromatography

(c) Use of molecular sieves

(d) Vacuum distillation

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The correct option is (b) Chromatography

The explanation: The methodologies used to remove the excess water present are as follows:

• Use of hydrolases.

• Use of molecular sieves which absorbs water.

• Vacuum distillation, if the organic phase has a high boiling point.

Chromatography is used for separation of a mixture which is dissolved in a fluid called the mobile phase and carrying it through a structure holding another material called the stationary phase. The separation is based on differential partitioning between the mobile and stationary phases.

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