Question

For immobilized enzymes, the volumetric surface area (A/V) is an important parameter which does not govern which of the following?

(a) The overall flux of the substrate to the biocatalytic surface

(b) Effectiveness of the enzyme

(c) Recovery of the biocatalyst

(d) Productivity

This question was addressed to me by my school teacher while I was bunking the class.

Query is from Recent Advances in chapter Recent Advances in Enzyme Technology of Enzyme Technology

Answer 1

The correct option is (c) Recovery of the biocatalyst

The best I can explain: For immobilized enzymes, the following things are governed by the volumetric surface area (A/V) is an important parameter:

• The overall flux of the substrate to the biocatalytic surface,

• The effectiveness of the enzyme and

• productivity.

Recovery of the biocatalyst is an advantage of impregnated wet beads along with some others which are as follows:

• Protection of the contained enzyme at higher rates of stirring.

• Allows to be used with low molecular weight hydrophilic coenzymes with the assistance of a coenzyme regenerating process.

• Allowing efficient and continuous use of biphasic PBRs as long as the moving organic phase is saturated with water.