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Which of the following factor is not important for making the choice of organic solvents in biphasic system?

(a) Solvent polarity

(b) Ease of recovery

(c) Reproducible and linear

(d) Specific inhibitory effects

I had been asked this question during an online interview.

Origin of the question is Recent Advances topic in portion Recent Advances in Enzyme Technology of Enzyme Technology

1 Answer

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Correct choice is (c) Reproducible and linear

Explanation: The choice of organic phase depends on the following factors

* Solvent polarity

* Cost

* ease of recovery

* fire and fume hazards

* specific inhibitory effects

The response should be accurate, precise, reproducible and linear over the useful analytical range, without dilution or concentration. It should also be free from electrical noise. This is a feature that a biosensor should possess.

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