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It would be useful if enzyme catalyzed reactions where performed in organic solvents, other than water.

(a) True

(b) False

This question was posed to me during an online exam.

The doubt is from Recent Advances topic in chapter Recent Advances in Enzyme Technology of Enzyme Technology

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The correct option is (a) True

The explanation is: It would be useful if enzyme catalyzed reactions where performed in organic solvents, other than water because of the following reasons:

• Water is not the ideal medium for the majority of organic reactions.

• Many reactants such as molecular oxygen, steroids and lipids are more soluble in organic solvents than in water.

• For accomplishing reactions with reactants quite labile in an aqueous environment, non-aqueous media may be used which allows a much increased volumetric activity to be achieved.

• Microbial contamination is a lesser of a problem in organic solvents. As a result of this, microbial proteases are absent which leads to an apparent stabilization in the biocatalyst.

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