Question

Which of this is true for optimizing a two-phase system for enzyme-catalyzed reactions?

(a) Protection of the contained enzyme

(b) More facile recovery of the biocatalyst

(c) The difference between the Log P values of the substrates and the interphase should be as small as possible

(d) Allowing efficient and continuous use of biphasic PBRs

I had been asked this question in an international level competition.

Question is from Recent Advances in division Recent Advances in Enzyme Technology of Enzyme Technology

Answer 1

The correct answer is (c) The difference between the Log P values of the substrates and the interphase should be as small as possible

Explanation: The optimization of the two-phase system for enzyme-catalyzed reactions depends upon the concept of Log P being extended to cover the substrates, products and the interphase. The difference between the Log P values of the substrates and the interphase should be as small as possible, whereas the Log P values is much lower between substrates and organic phase. This leads to high concentrations of substrates within the interphase, and in turn transferring of a substrate from the organic phase into the aqueous phase.