Question

Which of this is a disadvantage of aqueous 2 phase system?

(a) The enzymes which enter the upper phase are gradually lost

(b) The enzymes are largely retained in homogeneous solution

(c) The enzymes are stabilized by the presence of the polymers in homogeneous solution

(d) No diffusion limitations to mass transfer exist

I had been asked this question in exam.

The doubt is from Recent Advances topic in section Recent Advances in Enzyme Technology of Enzyme Technology

Answer 1

Correct choice is (a) The enzymes which enter the upper phase are gradually lost

The best I can explain: Advantages of aqueous 2 phase system are as follows:

* The enzymes are largely retained in homogeneous solution.

* The enzymes are stabilized by the presence of the polymers in homogeneous solution.

* No diffusion limitations to mass transfer exist.

Disadvantages of aqueous 2 phase system are as follows:

* The need to separate the product from the upper phase polymers.

* The enzymes which enter the upper phase are gradually lost.