Question

__________________ refers to the process of separating or extracting the target compound from other compounds or contaminants.

(a) Quantification of a compound

(b) Identification of a compound

(c) Purification of a compound

(d) Chemical separations

I have been asked this question during an interview for a job.

The above asked question is from Enzymes Purification in section Enzymes Purification of Enzyme Technology

Answer 1

The correct choice is (c) Purification of a compound

Explanation: The process of separating or extracting the target compound from other compounds or contaminants is referred to as purification. Chromatography produces characteristic peaks for a specific compound. Hence the target compounds, contaminants and other compounds must have different peaks which means they must have different migration values so that the desired product may be eluted, and not the undesired ones.