Question

On spraying the coloring agent, the enzyme is detected as a __________

(a) square

(b) triangle

(c) line

(d) spot

I had been asked this question by my school teacher while I was bunking the class.

I'd like to ask this question from Enzymes Purification in chapter Enzymes Purification of Enzyme Technology

Answer 1

The correct choice is (d) spot

To explain: After performing the TLC, the plate is sprayed with coloring agent and the protein or enzyme is detected as spot. The coloring agent used is ABTS which can detect laccase enzyme.