Question

When protease depolymerize protein, the extent of hydrolysis is described in ________ units.

(a) Vmax

(b) Km

(c) DH

(d) Kcat

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Question is from Large Scale Use of Enzyme in portion Large Scale Use of Enzyme in Solution of Enzyme Technology

Answer 1

The correct choice is (c) DH

For explanation: When protease is used to depolymerize the protein, the extent of hydrolysis (degree of hydrolysis) is described in DH units and is given by

DH=\(\frac{Number \,of \,peptide \,bond \,cleaved}{Initial \,number \,of \,peptide \,bonds \,present}\)

Commercially, using enzymes such as subtilisin, DH values of up to 30 are produced using protein preparations of 8-12% (w/w).