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When protease depolymerize protein, the extent of hydrolysis is described in ________ units.

(a) Vmax

(b) Km

(c) DH

(d) Kcat

This question was addressed to me in unit test.

Question is from Large Scale Use of Enzyme in portion Large Scale Use of Enzyme in Solution of Enzyme Technology

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The correct choice is (c) DH

For explanation: When protease is used to depolymerize the protein, the extent of hydrolysis (degree of hydrolysis) is described in DH units and is given by

DH=\(\frac{Number \,of \,peptide \,bond \,cleaved}{Initial \,number \,of \,peptide \,bonds \,present}\)

Commercially, using enzymes such as subtilisin, DH values of up to 30 are produced using protein preparations of 8-12% (w/w).

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