Question

_________________ is not used in starch hydrolysis.

(a) Rennet

(b) Sachharifying α – amylase

(c) β – amylase

(d) Glucoamylase

This question was posed to me in a job interview.

My doubt stems from Large Scale Use of Enzyme in section Large Scale Use of Enzyme in Solution of Enzyme Technology

Answer 1

The correct option is (a) Rennet

The best explanation: Rennet is not used in starch hydrolysis. It is a proteolytic enzyme that is used in the production of cheese to coagulate casein. Saccharifying α – amylase, β – amylase, Glucoamylase obtained from Bacillus Subtilis, malted barley and Aspergillus Niger respectively are used in hydrolysis of starch. Saccharifying α – amylase cleave only α -1,4-oligosaccharide giving rise α -dextrins with maltose, G3, G4 and glucose. β – amylase cleaves α -1,4 links from non-reducing end to give limit dextrins and β – maltose. Glucoamylases cleaves α – 1,4 and α – 1,6 links to give β-glucose.