Question

Which of the following enzyme hydrolysis casein?

(a) Papain

(b) Rennet

(c) Alkaline protease

(d) Trypsin

This question was addressed to me during an online interview.

I'd like to ask this question from Large Scale Use of Enzyme in section Large Scale Use of Enzyme in Solution of Enzyme Technology

Answer 1

The correct option is (b) Rennet

For explanation: Rennet hydrolyzes the peptide linkage between phenylalanine and methionine residues in the casein protein present in the milk. This results in the release of a hydrophilic oligopeptide and the hydrophobic para-k-casein which removes the protective effect and allows coagulation of the milk to form curds, which are then compressed and turned into cheese.