Question

Which of the following is not true for radioisotope method?

(a) This method is used in studying mechanism of enzyme action and in ligand binding sites

(b) Change is absorbance is used as the basis for assaying enzyme

(c) If one product is gas, the method is easier

(d) Glutamate decarboxylase is assayed and measured using this method

This question was posed to me during an interview for a job.

I would like to ask this question from Enzyme Assay Techniques topic in division Enzyme Assay & Enzyme Catalysis of Enzyme Technology

Answer 1

The correct answer is (b) Change is absorbance is used as the basis for assaying enzyme

The best I can explain: “Change is absorbance is used as the basis for assaying enzyme.” This statement is true for spectrophotometer. Following statements are true for radioisotope method:

• This method is used in studying mechanism of enzyme action and in ligand binding sites.

• If one product is gas, the method is easier.

• Glutamate decarboxylase is assayed and measured using this method.