Question

Which of the following is not true for spectrofluorometric method?

(a) The intensity of emitted light is used to study enzyme reaction

(b) Trace impurities in enzyme can stop the emitted radiation

(c) It is based on stoke shift

(d) Hydrolases can be assayed and measured by using this method

I got this question by my school principal while I was bunking the class.

This is a very interesting question from Enzyme Assay Techniques in division Enzyme Assay & Enzyme Catalysis of Enzyme Technology

Answer 1

Right choice is (a) The intensity of emitted light is used to study enzyme reaction

The best I can explain: “The intensity of emitted light is used to study enzyme reaction.” This is true of luminescence method and not spectrofluorometric method. Following statements are true for spectrofluorometric method are as follows:

• Trace impurities in enzyme can stop the emitted radiation.

• It is based on stoke shift. The increase in wavelength due to absorption in UV region and emission in visible light is known as stoke shift.

• Hydrolases can be assayed and measured by using this method.