Question

Restriction site can also be introduced by oligonucleotide directed mutagenesis of a region that is _______   and ______ to the original restriction site.

(a) not only similar, also exactly same

(b) similar, not same

(c) not similar, is entirely different

(d) different, near

This question was addressed to me in my homework.

Question is from Removal and Introduction of Restriction Sites and Generation of Insertions and Deletions topic in chapter Modifications and Mutagenesis of Genetic Engineering

Answer 1

Right answer is (b) similar, not same

The explanation is: Restriction site can also be introduced by oligonucleotide directed mutagenesis of a region that is similar and is not the same to the original restriction site.